FastQCFastQC Report
Wed 25 May 2016
SRR1779654_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779654_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences563059
Sequences flagged as poor quality0
Sequence length76
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGT57531.0217401728770874No Hit
CATATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGT23170.41150216939965445No Hit
TCTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCG14860.26391550441427986No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG12590.22360001349769743No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCC6290.11171120610806327No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG10750.052.73468470
TCGTATG16400.048.44540
CTCGTAT16350.047.51859339
TTGAAAA16750.047.2279658
GTATGCC16300.047.06204242
CGTATGC16450.047.0504141
CTTGAAA16450.047.02533357
TGAAAAA17650.046.00963659
TCTCGTA16900.045.76505338
GTCTTCT16800.044.38671549
CCGTCTT16600.044.31241647
TATGCCG17300.044.13921743
GAAAAAA19400.043.836260
GCTTGAA17500.043.8076756
TCTTCTG17300.043.5085950
TTCTGCT17400.043.45974352
TCTGCTT17500.043.0151753
CTTCTGC17700.042.12976551
GCCGTCT17550.042.1133346
ATATACA6850.041.8902632