FastQCFastQC Report
Wed 25 May 2016
SRR1779653_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779653_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences237625
Sequences flagged as poor quality0
Sequence length76
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG200108.420831141504472No Hit
CTTATACACATCTGACGCTGCCGACGATATGCAGTGTGTAGATCTCGGTG9520.4006312467122567No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTTTCG150.002222726670.0000158
TACTGAT200.006945286452.52
TATAGAC200.006945286452.53
GACGATT200.006945286452.514
GCCGTAT1800.046.66666454
TAGATCT2050.044.39024439
AGATCTC2150.042.3255840
CGTATCA2000.042.056
CCGTATC2000.042.055
GTAGATC2250.040.44444738
ACGGCAA350.001253759440.00000411
ATCTCGG2300.039.5652242
TGGTCGC2250.037.33333249
TCTCGGT2300.036.5217443
GTATCAT2300.036.5217457
CGCCGTA2250.035.7777853
ATCATTA2350.035.74468259
TTGTCTG601.2124961E-535.0000049
AGTTTGT400.00241558635.023
GAGCTAA400.00241558635.01
Produced by FastQC (version 0.11.5)