FastQCFastQC Report
Wed 25 May 2016
SRR1779653_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779653_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences237625
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGT75983.197475013150973No Hit
CATATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGT28211.1871646501841135No Hit
TCTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCG19570.8235665439242503No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG16720.703629668595476No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCC6570.27648605996843767No Hit
CATAAACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGT3870.1628616517622304No Hit
CTTAAACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGT3640.15318253550762756No Hit
TCTTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCC2970.12498684902682798No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTAT150.002211927470.0859946
AACGTTT150.002224900869.9827516
AGGGGGG14700.059.50913670
ACGTATG252.390075E-455.9861940
GCCGTAA200.006911639652.56448746
TGACGTA200.00691740352.55341345
CTTGACT200.006928941252.53127349
AATCGTA200.00695206152.48705738
CGTATGC23300.051.55430241
TATGCCG22400.051.29133643
GTATGCC22700.050.92209642
TCGTATG24350.050.8704140
CTCGTAT23750.050.6822439
CCGTCTT21900.050.5643247
TCTCGTA24050.050.3410238
ATGCCGT22850.050.2812244
CGTCTTA352.067357E-550.0614248
AATCTCG24400.050.04913736
GTCTTCT23350.049.79409849
GCCGTCT22550.049.72841646