FastQCFastQC Report
Wed 25 May 2016
SRR1779652_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779652_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences390738
Sequences flagged as poor quality0
Sequence length76
%GC64

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG187584.800659265287738No Hit
CTTATACACATCTGACGCTGCCGACGATATGCAGTGTGTAGATCTCGGTG8110.20755595821240833No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCCCT200.006948620552.534
AGTGTAG200.006948620552.52
TATCATT1150.051.73913258
TCATTAA1250.050.39999860
CATTAAA1300.048.4615461
ATCATTA1400.047.559
CGTATCA1350.046.66666456
ATTAAAA1550.040.6451662
TTAAAAA1550.040.6451663
TATTGAC350.001254751740.02
ATACCGT459.1907496E-538.888896
CCGTATC1550.038.38709655
GTATCAT1550.038.38709657
GCCGTAT1650.038.1818254
TATAAGA753.5319317E-837.3333322
TGTTTCG400.00241748635.026
CTGTAAT400.00241748635.014
GTATAAG851.0619442E-732.9411771
TAGATCT2150.032.5581439
TGTGTAG2300.031.95652435
Produced by FastQC (version 0.11.5)