FastQCFastQC Report
Wed 25 May 2016
SRR1779652_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779652_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences390738
Sequences flagged as poor quality0
Sequence length76
%GC62

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGT74221.8994825176972805No Hit
CATATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGT26420.6761564014761811No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCG16700.42739636278017495No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG14360.3675096867978031No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCC6650.17019076721486007No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACGTA150.00222468969.99296638
AACTAGG150.002225818669.984013
AATATAC700.059.9862861
AGGGGGG12050.057.49722770
TCTTTAT509.822543E-1055.98721
TTAGGGC200.006954903752.4880034
TAATACG200.006954903752.4880034
CTACTGT200.006954903752.4880033
GGTAAAT200.006954903752.48800363
GTGCTTA200.006954903752.4880036
ATATACA6550.051.8202172
CATATAC5900.050.4122051
ACGTATG707.2759576E-1250.0013740
TGATAAG352.0861637E-549.99497230
ATTGCTA352.0861637E-549.99497228
TGAAAAA22800.049.89171659
GAAAAAA23700.049.61526560
TTGAAAA21900.049.5447458
CTTGAAA21600.049.2669257
TCGTATG21700.049.19489740
Produced by FastQC (version 0.11.5)