FastQCFastQC Report
Wed 25 May 2016
SRR1779651_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779651_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences517591
Sequences flagged as poor quality0
Sequence length76
%GC63

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG166583.218371262251469No Hit
CTTATACACATCTGACGCTGCCGACGATATGCAGTGTGTAGATCTCGGTG6330.12229733515459118No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATTTG150.002224211870.066
TTAGCTT200.00694988852.54
TACGTAA200.00694988852.529
TAGGACA459.1948896E-538.8888854
TCATTAA1200.037.91666460
CATTAAA1300.037.69230761
TATCATT1350.036.29629558
TTTCACA400.002418208435.02
ATTAAAA1550.033.87096862
ATCATTA1350.033.70370559
GTCCTAT450.00430629531.11110964
CGTATCA1600.030.62556
TTAAAAA1850.030.27027163
GTATCAT1750.030.057
GTGTATA604.980553E-429.1666661
TATAAGA1202.2955646E-929.1666662
CTGCACT853.953468E-628.823534
TAGATCT1750.028.039
CTCGTTT500.007206765527.99999846
ATATACG500.007206765527.9999982
Produced by FastQC (version 0.11.5)