FastQCFastQC Report
Wed 25 May 2016
SRR1779651_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779651_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences517591
Sequences flagged as poor quality0
Sequence length76
%GC62

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGT53331.0303502186089017No Hit
CATATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGT18240.35240179987673664No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG12540.24227623741525645No Hit
TCTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCG11850.22894524827518253No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTTAA401.3460522E-1070.02949
TCTTAAG401.0621079E-861.27537550
TTTATAC401.0670192E-861.2398573
CTTTATA401.0670192E-861.2398572
CCTTATA252.3917612E-455.990731
GTTACAC252.3917612E-455.990733
AGGGGGG8000.055.55330370
TGTTAAC200.00693055852.53698747
TAATAAG200.006938512452.52174850
GTATTAT200.006938512452.52174849
ACGTTAC200.00695444252.4913021
ATAGTAT200.00695444252.49130239
ACCCAAT200.00695444252.4913021
TAGTATG1550.051.92688440
TCTTTAT506.200753E-848.9918861
CGTCTTA1600.048.15890548
TCGTATG14550.047.86149240
AGTATGC1250.047.6105141
TGAAAAA17600.047.53431759
GTATGCC14900.046.99933242
Produced by FastQC (version 0.11.5)