FastQCFastQC Report
Wed 25 May 2016
SRR1779650_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779650_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences535442
Sequences flagged as poor quality0
Sequence length76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG192003.585822554076819No Hit
CTTATACACATCTGACGCTGCCGACGATATGCAGTGTGTAGATCTCGGTG12460.2327049428322769No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA2900.043.44827756
CCGTATC3150.040.055
GTATCAT3200.039.37557
TAGATCT3700.038.78378339
GCCGTAT3350.038.6567254
GTAGATC4000.034.12538
AGATCTC4150.033.7349440
CATTAAA3850.033.63636461
ATCATTA3750.033.60000259
TATCATT3900.032.30769358
TCATTAA4150.032.04819560
TGTGTAG4000.031.49999835
TGTAGAT4350.031.3793137
CGCCGTA4150.031.2048253
TCTCGGT4450.029.8876443
GTGTGTA4300.029.30232634
CGGTGGT4700.029.04255346
AGTGTGT4350.028.16092133
TCGGTGG4850.028.1443345
GCGTTAT500.007206984327.99999811