FastQCFastQC Report
Wed 25 May 2016
SRR1779650_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779650_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences535442
Sequences flagged as poor quality0
Sequence length76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGT74221.3861445310603202No Hit
CATATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGT25880.4833389984349378No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20450.38192745432745284No Hit
TCTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCG18140.3387855267237161No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCC6850.12793169008034483No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG13350.054.26119270
TCGTATG21400.049.23048440
CGTATGC20700.049.04906541
CTCGTAT21600.048.61260639
GTATGCC20700.048.54619642
TCTCGTA22600.048.00583638
CCGTCTT19000.047.93202647
TATGCCG21100.047.29842443
TTATCTC22400.046.71145635
GATTATC23100.046.05341733
CGTCTTC19600.045.74987448
CTTGAAA21450.045.68904557
GCCGTCT20200.045.25797746
GTCTTCT19150.045.15764249
ATCTCGT23950.045.15374437
ATTATCT23400.045.0143434
ATGCCGT22000.044.8859844
TATCTCG23850.044.46261636
TGAAAAA25400.043.95804259
TTGAAAA22900.043.7131358