FastQCFastQC Report
Wed 25 May 2016
SRR1779649_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779649_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences348060
Sequences flagged as poor quality0
Sequence length76
%GC60

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG174995.027581451473884No Hit
CTTATACACATCTGACGCTGCCGACGATATGCAGTGTGTAGATCTCGGTG7830.22496121358386487No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTAAC252.3888829E-455.99921
TACGGTT200.006948380752.4992548
TTGTATA350.001254651639.99942853
GTACTAC350.001254651639.9994281
GCCGTAT1600.032.8120354
CAATTTG450.004304678631.11066230
GTATAGA450.004304678631.1106621
CCGTATC1600.030.62456155
CGTATCA1401.2732926E-1129.9995756
CATGCTA604.977659E-429.16624814
GTAGGAT604.977659E-429.1662483
TAGATCT1950.026.92269139
ATCATTA1455.9117156E-1026.55134259
GTATCAT1606.548362E-1126.24962457
TATCATT1508.767529E-1025.66629658
ATCCCTA851.3053126E-424.70907811
GTAGATC2150.024.41825538
CCTCTCA1151.491142E-624.3509759
CGCCGTA2300.024.34747753
TGTAGAT2450.024.28536637
Produced by FastQC (version 0.11.5)