FastQCFastQC Report
Wed 25 May 2016
SRR1779649_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779649_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences348060
Sequences flagged as poor quality0
Sequence length76
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGT69812.0056886743664886No Hit
CATATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGT23380.6717232661035454No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCG15990.4594035511118773No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG14260.40969947710164917No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCC6700.19249554674481412No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG11750.059.2650470
CGGGTTA200.00695334452.48976519
GATCCTA200.00695334452.4897651
TAAGCGC200.00695334452.48976537
TCGTATG20850.052.3638940
CGTATGC19950.052.30056441
CTCGTAT21050.051.7001339
GTATGCC19500.051.367642
CTTGAAA19600.051.07614557
TCTCGTA21500.050.94355438
CCGTCTT18100.050.52509747
GTCTTCT18450.050.47987449
TCTTCTG18400.050.42675850
TATGCCG19950.050.39173543
TTGAAAA20250.050.30094558
TGAAAAA22150.050.2529559
TCTGCTT18150.050.1712153
GAAAAAA23900.049.7953960
CGTCTTC18800.049.5757248
GCCGTCT18450.049.55949846
Produced by FastQC (version 0.11.5)