FastQCFastQC Report
Wed 25 May 2016
SRR1779648_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779648_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences224928
Sequences flagged as poor quality0
Sequence length76
%GC67

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG205959.156263337601365No Hit
CTTATACACATCTGACGCTGCCGACGATATGCAGTGTGTAGATCTCGGTG10920.48548868971404185No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACAGA150.002222571570.000016
TATTCAG150.002222571570.000012
TCTTGGA200.00694480652.52
ATTACAC200.00694480652.53
CTCCAAT200.00694480652.547
TACGCTC200.00694480652.559
ACAGAAT200.00694480652.53
CTATTAC200.00694480652.51
TATGTGC200.00694480652.550
ATTGAGA200.00694480652.530
ATCATTA1750.050.059
TCATTAA1850.049.1891960
CATTAAA1950.046.66666861
AGCGGTA305.8692653E-446.66666859
TATCATT1900.046.05263558
GTATCAT1950.044.87179657
ATTAAAA2150.043.95348762
CGTATCA2000.043.74999656
TTAAAAA2200.041.36363663
TAGATCT2250.040.44444739
Produced by FastQC (version 0.11.5)