FastQCFastQC Report
Wed 25 May 2016
SRR1779648_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779648_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences224928
Sequences flagged as poor quality0
Sequence length76
%GC63

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGT75873.373079385403329No Hit
CATATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGT33221.4769170579029733No Hit
TCTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCG20560.9140702802674634No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG17590.7820280267463366No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCC7370.327660406885759No Hit
CATAAACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGT3980.17694551145255372No Hit
CTTAAACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGT3850.1711658841940532No Hit
TCTTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCC2750.12226134585289515No Hit
CACTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCC2570.11425878503343292No Hit
ATTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGT2500.1111466780480865No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGGGG150.002217674470.0389346
GTATGAA150.002219632370.0233542
TAGCGTT150.00222355269.9922263
CGGTTAG150.00222355269.9922232
ATAGCGT150.00222355269.9922262
GTTAGTG150.00222355269.9922234
TTATCTT150.00222355269.9922269
TTTATAC301.0213262E-769.992223
TGTAGCA150.00222355269.9922216
CTAATAA207.919138E-569.992221
AGGGGGG12250.061.1360670
GAGAATA352.9674266E-759.99333224
CTTTATA352.9674266E-759.9933322
CGTATGA1000.059.5198541
CTAAAAC308.363795E-658.3268471
AGAATAC308.363795E-658.32684725
CATATAG252.3829566E-456.01868452
AGACTAA252.3882007E-455.9937825
GGTACCA252.3882007E-455.9937869
TCTAGTA252.3882007E-455.9937838
Produced by FastQC (version 0.11.5)