FastQCFastQC Report
Wed 25 May 2016
SRR1779647_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779647_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences636257
Sequences flagged as poor quality0
Sequence length76
%GC60

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG176022.766492156471363No Hit
CTTATACACATCTGACGCTGCCGACGATATGCAGTGTGTAGATCTCGGTG7380.11599086532643255No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACTAA400.002418624235.013
TATGATC552.991964E-431.8181844
GTATAAG1550.031.6129061
TCATTAA2000.031.50000260
CTATAAC450.0043070331.1111131
ATCATTA2050.030.73170959
CATTAAA2350.028.29787361
GTATCAT2350.028.29787357
ATTACAC500.007207987428.0000023
TATAAGA1850.026.4864862
CGTATCA2550.026.07843256
CCGTATC2450.025.71428755
TATCATT2600.025.57692358
GCCGTAT2450.024.28571554
TACTGTT750.001832135723.3333347
ATAAGAG1953.092282E-1123.3333343
TAGATGA901.9275559E-423.3333344
TAGATCT2850.023.33333239
AATTTTC800.00266585121.87546
TACTTCG800.00266585121.8755
Produced by FastQC (version 0.11.5)