FastQCFastQC Report
Wed 25 May 2016
SRR1779647_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779647_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences636257
Sequences flagged as poor quality0
Sequence length76
%GC60

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGT64831.0189278860586208TruSeq Adapter, Index 5 (95% over 23bp)
CATATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGT21440.33697075238464963TruSeq Adapter, Index 5 (95% over 23bp)
TCTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCG14650.2302528695165633TruSeq Adapter, Index 5 (95% over 22bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG13090.205734475219919No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG11500.054.16016870
TCGTATG19200.049.94309240
CGTATGC19250.049.29509441
GTATGCC19750.048.25097342
CTCGTAT19450.048.03785739
CTTGAAA19000.047.8901157
TGAAAAA21800.047.67888359
GAAAAAA23450.047.603660
TTGAAAA19450.047.3219158
GTCTTCT18250.046.83417549
TCTTCTG18350.046.76984450
TATGCCG20350.046.48401343
CCGTCTT18850.046.4730647
TCTCGTA20450.046.03105538
TCTGCTT18200.045.61554753
ACGTATG703.8380676E-1044.99565540
GCTTGAA19900.044.67256556
CGTCTTC19850.044.30837248
TGCTTGA19100.043.80535555
GCCGTCT20150.043.47137546
Produced by FastQC (version 0.11.5)