FastQCFastQC Report
Wed 25 May 2016
SRR1779646_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779646_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences409917
Sequences flagged as poor quality0
Sequence length76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG139153.3945896364385963No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTACGG552.9901197E-431.81818426
GTACATG450.004305260731.1111091
GGCCCTA450.004305260731.11110911
TAAGCTT450.004305260731.11110914
GCATATG500.00720504327.9999981
ATGTGGA657.950554E-426.9230772
ACAGTGC700.001225208625.08
GTTCTTA851.3070137E-424.7058831
GTGCCTA851.3070137E-424.70588311
TATGGTT1001.3959096E-524.4999984
TGTATGG750.001831028823.3333342
AATCTCG750.001831028823.33333431
GATACCT850.003786666620.58823628
GGGACGT850.003786666620.5882366
TGAACGG1205.7062047E-520.4166665
GGACGTG1408.230005E-620.07
CATGTAG1257.810695E-519.61
CTGTAAC900.005271190319.44444348
CCAATTT900.005271190319.44444316
AAGATAC900.005271190319.44444326
Produced by FastQC (version 0.11.5)