FastQCFastQC Report
Wed 25 May 2016
SRR1779646_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779646_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences409917
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGT76911.876233481412091No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCG17800.43423424742081934No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCC5600.1366130216604825No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATGG200.00694852652.50064539
CTCGTAT14550.050.27552839
TATGCCG14500.050.22588743
TCGTATG15350.050.1634840
AGGGGGG6450.049.91770
CGTATGC15200.049.7556641
GTATGCC14800.049.20779442
TCTCGTA14950.048.93036338
CTTGAAA15450.048.70609757
TTGAAAA16050.047.7575958
TGAAAAA16800.047.29224459
AATCTCG15850.047.25609636
CCGTCTT14700.047.16644747
ATCTCGT16100.046.0875237
GCTTGAA16150.045.9448356
CGTCTTC15400.045.93206448
TCTTCTG14900.045.8171150
GCCGTCT15400.045.70467846
GTCAATC16300.045.51647633
ATGCCGT15950.045.44038444
Produced by FastQC (version 0.11.5)