FastQCFastQC Report
Wed 25 May 2016
SRR1779645_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779645_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences155482
Sequences flagged as poor quality0
Sequence length76
%GC67

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG123917.9694112501768695No Hit
CGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1580.10161948006843236No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTTAAT150.002221276870.0000147
TAAAGCT200.00694078952.5000044
TCTGTGG200.00694078952.50000449
GTATAAG200.00694078952.5000041
TGTACAT200.00694078952.50000410
CTCTAAA400.002413024435.00000440
AGTACGG400.002413024435.0000042
TCTAAAA552.9816452E-431.8181841
AATCAGC450.004297119631.1111091
TTTCCGC450.004297119631.11110924
GTACGTG450.004297119631.1111091
TACGGCA604.964724E-429.1666682
AGAACAC604.964724E-429.1666685
TCTACAA500.00719150228.0000022
CACAAAT500.00719150228.00000252
ACATATG500.00719150228.00000260
CTAAAAC755.5574958E-527.99999842
TAAAACA657.9282455E-426.92307743
TCCAGCT808.62343E-526.2500022
TCCTTAC4000.026.25000270
Produced by FastQC (version 0.11.5)