FastQCFastQC Report
Wed 25 May 2016
SRR1779645_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779645_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences155482
Sequences flagged as poor quality0
Sequence length76
%GC63

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGT67474.3394090634285645No Hit
TCTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCG17021.0946604751675435No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCC4960.3190079880629269No Hit
ATTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGT2450.15757451023269572No Hit
TCTTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCC2010.1292754145174361No Hit
ATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGTCTT2000.1286322532511802TruSeq Adapter, Index 2 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGACGT1810.11641218919231809No Hit
CTTATACACATCTCCGAGCCCACGAGACGCGCACGTATCTCGTATGCCGT1650.10612160893222367No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAATCT150.002219656770.0128749
TATTCTG252.826353E-670.0128750
GTATATC252.8317627E-669.9903635
TTGGACC150.002222491769.9903525
AACCCGT150.002222491769.9903517
TTTCAGA150.002222491769.9903524
AGTCTTC550.063.6890548
CGTCTTA401.0522854E-861.3007148
ACGTCTT700.060.0496747
TCTTTAT352.9553667E-760.011031
CGTATCA308.353014E-658.3252934
CCCGTAT308.353014E-658.3252932
CTAGTAT308.353014E-658.3252939
TAGTATG308.353014E-658.3252940
AGGGGGG5050.058.20979370
GTATGCA750.056.0463642
AGTATGC252.3824212E-456.01029641
GTATGAG252.3824212E-456.0102961
TCTAGTA252.3862132E-455.99228338
TATGCAG700.055.04553243
Produced by FastQC (version 0.11.5)