FastQCFastQC Report
Wed 25 May 2016
SRR1779644_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779644_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences235400
Sequences flagged as poor quality0
Sequence length76
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG120105.1019541206457095No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTATCG150.002222700770.000015
AATTTAC200.006945205452.567
TACGGTA200.006945205452.556
TACGTAG200.006945205452.546
GGGTTAT305.869827E-446.6666681
TTTACTA350.001253735740.0000042
TTCATAC552.98628E-431.8181852
TTTAGCA450.004301571831.11111318
TACTACA450.004301571831.1111132
GGTAGAC450.004301571831.11111345
GCTTTCT450.004301571831.1111131
AAGACGG604.972402E-429.1666685
AAAGGTC604.972402E-429.16666823
AGCAATC500.00719890927.9999985
TTAGCAT500.00719890927.99999819
CGACTCA500.00719890927.99999870
CCAGTTC500.00719890927.99999813
TTGGGCA500.00719890927.99999853
ATGTAAA657.9404464E-426.92307739
ACAGACG657.9404464E-426.92307766
Produced by FastQC (version 0.11.5)