##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1779644_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 235400 Sequences flagged as poor quality 0 Sequence length 76 %GC 59 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.117956669498724 32.0 27.0 32.0 13.0 32.0 2 29.000450297366186 32.0 32.0 32.0 13.0 32.0 3 28.59803738317757 32.0 27.0 32.0 13.0 32.0 4 27.457769753610876 32.0 27.0 32.0 13.0 32.0 5 28.502744265080715 32.0 27.0 32.0 13.0 32.0 6 32.23752761257434 37.0 32.0 37.0 13.0 37.0 7 32.0229056924384 37.0 32.0 37.0 13.0 37.0 8 32.37459643160578 37.0 32.0 37.0 13.0 37.0 9 32.400807136788444 37.0 32.0 37.0 13.0 37.0 10-11 31.95823491928632 37.0 32.0 37.0 13.0 37.0 12-13 31.86344732370433 37.0 32.0 37.0 13.0 37.0 14-15 32.85775063721326 37.0 34.5 37.0 17.5 37.0 16-17 32.89246389124894 37.0 34.5 37.0 17.5 37.0 18-19 32.14000212404418 37.0 32.0 37.0 13.0 37.0 20-21 32.65263381478334 37.0 34.5 37.0 17.5 37.0 22-23 32.597425658453695 37.0 32.0 37.0 17.5 37.0 24-25 31.94200084961767 37.0 32.0 37.0 13.0 37.0 26-27 32.586918011894646 37.0 32.0 37.0 17.5 37.0 28-29 32.0024787595582 37.0 32.0 37.0 13.0 37.0 30-31 32.14870433305013 37.0 32.0 37.0 13.0 37.0 32-33 32.10768266779949 37.0 32.0 37.0 13.0 37.0 34-35 32.01811172472388 37.0 32.0 37.0 13.0 37.0 36-37 32.787325828377234 37.0 34.5 37.0 17.5 37.0 38-39 32.62020178419711 37.0 32.0 37.0 17.5 37.0 40-41 31.92646134239592 37.0 29.5 37.0 13.0 37.0 42-43 31.936495327102804 37.0 32.0 37.0 13.0 37.0 44-45 31.553952846219204 37.0 32.0 37.0 13.0 37.0 46-47 31.94048428207307 37.0 29.5 37.0 13.0 37.0 48-49 32.51398045879354 37.0 32.0 37.0 13.0 37.0 50-51 32.7692417162277 37.0 32.0 37.0 13.0 37.0 52-53 31.619923534409516 37.0 29.5 37.0 13.0 37.0 54-55 31.821529311809684 37.0 29.5 37.0 13.0 37.0 56-57 31.581125743415463 37.0 29.5 37.0 13.0 37.0 58-59 31.073793542905694 37.0 27.0 37.0 13.0 37.0 60-61 30.863266779949022 37.0 27.0 37.0 13.0 37.0 62-63 30.545038232795243 37.0 27.0 37.0 10.5 37.0 64-65 30.487909940526762 37.0 27.0 37.0 10.5 37.0 66-67 30.534396771452847 37.0 27.0 37.0 10.5 37.0 68-69 30.93195624468989 37.0 27.0 37.0 13.0 37.0 70-71 30.991892523364484 37.0 27.0 37.0 13.0 37.0 72-73 31.39341121495327 37.0 32.0 37.0 13.0 37.0 74-75 31.79085386576041 37.0 32.0 37.0 13.0 37.0 76 31.227404418011893 37.0 27.0 37.0 13.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 11101 1 0.7060347540885523 11101 2 0.2493358430146948 11101 3 0.060921115584108065 11101 4 -0.21540177009873318 11101 5 -0.5097629643210837 11101 6 1.2629484368923016 11101 7 -0.681718871015569 11101 8 0.6066827755437174 11101 9 -1.414611142711724 11101 10-11 -0.5748608647250713 11101 12-13 -0.6677603177210969 11101 14-15 0.28151517701692796 11101 16-17 -1.1845325628475862 11101 18-19 0.44204970214720163 11101 20-21 0.32368168035604583 11101 22-23 0.3729016758311161 11101 24-25 0.8308207043439353 11101 26-27 0.30081938323406376 11101 28-29 0.1789216977353476 11101 30-31 0.4334392166613128 11101 32-33 0.8338624228450442 11101 34-35 -1.771399859472865 11101 36-37 -0.03604322409330507 11101 38-39 0.1399299494425179 11101 40-41 0.4615890962440119 11101 42-43 0.3102970813823056 11101 44-45 -0.3730982689449611 11101 46-47 0.2125119206039905 11101 48-49 -0.6577719434106726 11101 50-51 0.11506331788098123 11101 52-53 -0.5257667037969931 11101 54-55 0.21176070542289338 11101 56-57 0.08962046221377307 11101 58-59 -0.24899306622853956 11101 60-61 -0.2688383536301231 11101 62-63 0.5050129313586638 11101 64-65 -0.047458445157342766 11101 66-67 0.5246916435544335 11101 68-69 0.1917969121899752 11101 70-71 -0.18007469270461485 11101 72-73 0.23513500950207344 11101 74-75 -0.018450229958169473 11101 76 -1.216040459619606 11102 1 0.44527796434183387 11102 2 -0.7537920178336428 11102 3 0.1125689642261598 11102 4 0.06557168122870038 11102 5 -1.5440168550748226 11102 6 0.6220421201391773 11102 7 0.3185862861403663 11102 8 0.023756318418293176 11102 9 -1.025688347472176 11102 10-11 -1.3301629703094484 11102 12-13 -0.6499086240988774 11102 14-15 -0.05495873204623791 11102 16-17 -0.07013677401633345 11102 18-19 -0.7022514879657074 11102 20-21 -0.23590025434032214 11102 22-23 -0.6296158707053507 11102 24-25 0.7783336735230648 11102 26-27 -0.9537961187494588 11102 28-29 -0.6097951164239461 11102 30-31 -1.1135168407082325 11102 32-33 0.01867322540836369 11102 34-35 -0.23981609383356073 11102 36-37 -0.7507594279382843 11102 38-39 -0.7074151833008457 11102 40-41 -0.8471536562735338 11102 42-43 -0.7928841819683221 11102 44-45 -0.7804830721185958 11102 46-47 -0.8104893000246349 11102 48-49 -1.3285455168009683 11102 50-51 -1.4939922207214025 11102 52-53 -0.13409749415402317 11102 54-55 -0.17296617892254673 11102 56-57 -1.28385375200655 11102 58-59 -1.256850862993872 11102 60-61 -0.9343479660805372 11102 62-63 -1.559070071387751 11102 64-65 -0.8937355278549326 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11104 24-25 -1.2663974932799427 11104 26-27 -0.11286505605889374 11104 28-29 -0.5133051930209547 11104 30-31 -0.3959218381054228 11104 32-33 -1.41305875942097 11104 34-35 -1.7215592335673335 11104 36-37 -0.9834135515346247 11104 38-39 -1.4125910589608424 11104 40-41 -0.3334703068275644 11104 42-43 -1.0959257525788644 11104 44-45 -1.04507740542714 11104 46-47 -1.2824533068663122 11104 48-49 -0.5502306510316473 11104 50-51 -0.8948251202528965 11104 52-53 -1.4270996507688842 11104 54-55 -0.03215410187933898 11104 56-57 -0.8414502419299552 11104 58-59 -2.447413813837926 11104 60-61 -1.956211578782252 11104 62-63 0.04905813564727879 11104 64-65 -0.17242222377223726 11104 66-67 -0.20082270011407033 11104 68-69 -0.788607318467804 11104 70-71 -0.16699877268143482 11104 72-73 0.6002103499830937 11104 74-75 -0.5973766281036355 11104 76 -0.9982050495935262 11105 1 0.0627575554785551 11105 2 -0.9739333363491092 11105 3 -0.9852563770649176 11105 4 -0.2793552584708259 11105 5 0.06728862616274611 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20-21 0.24147407281961364 21611 22-23 -0.21010578950838266 21611 24-25 0.19013460409509264 21611 26-27 0.3346355936855403 21611 28-29 0.636395924539471 21611 30-31 0.2528046628042233 21611 32-33 0.80306789742734 21611 34-35 0.31214084440200196 21611 36-37 0.11517433722912784 21611 38-39 0.18889902160746885 21611 40-41 -0.06631705377375852 21611 42-43 0.9605370387232206 21611 44-45 -0.2679614043875489 21611 46-47 0.23610772579417372 21611 48-49 0.5135309360774158 21611 50-51 1.034677608673725 21611 52-53 -0.15836535292603315 21611 54-55 0.4660266137058606 21611 56-57 0.8487299538597028 21611 58-59 0.09938944354750134 21611 60-61 -0.3937494805408974 21611 62-63 0.07859984923992869 21611 64-65 0.7946140752834694 21611 66-67 0.8043752553567778 21611 68-69 0.3367933572664086 21611 70-71 0.39960003178880754 21611 72-73 -0.06618919952743241 21611 74-75 0.6995998944641606 21611 76 0.9591283992499271 21612 1 0.21172263179918005 21612 2 0.3070144779240458 21612 3 -0.6645499117888036 21612 4 -0.7282883680368784 21612 5 -0.042557134246429484 21612 6 1.0051276050401867 21612 7 -0.016237178871946867 21612 8 1.0532700489173337 21612 9 0.6490219891759423 21612 10-11 0.3404875177847053 21612 12-13 -0.3247101932987704 21612 14-15 -0.027452117686237898 21612 16-17 0.6397209032028996 21612 18-19 -0.22054361459648675 21612 20-21 0.3368348975618787 21612 22-23 0.16360555069780247 21612 24-25 0.3628150164662216 21612 26-27 0.32690363492265817 21612 28-29 -0.0337071682440353 21612 30-31 -0.13637059492774029 21612 32-33 0.024717381963419882 21612 34-35 0.49770785471127965 21612 36-37 -0.4002895802966435 21612 38-39 0.5548784030507647 21612 40-41 -0.23642014655726484 21612 42-43 0.3780628119190972 21612 44-45 0.9176056059217252 21612 46-47 0.5737365917735566 21612 48-49 0.464561863912472 21612 50-51 -0.26171414390358905 21612 52-53 0.08905732748633355 21612 54-55 0.08200599514916362 21612 56-57 -0.6641566440784459 21612 58-59 0.24887397962997326 21612 60-61 -0.195295872293471 21612 62-63 0.1481874781059105 21612 64-65 0.09616046703604297 21612 66-67 -0.37861443536487016 21612 68-69 -0.12196952933153327 21612 70-71 0.33516704209808523 21612 72-73 0.04463554274060755 21612 74-75 0.16094010064972153 21612 76 -0.07179943580161563 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 9.0 12 36.0 13 106.0 14 184.0 15 300.0 16 474.0 17 665.0 18 898.0 19 1300.0 20 2015.0 21 2804.0 22 3891.0 23 5007.0 24 5872.0 25 6615.0 26 7626.0 27 8521.0 28 9497.0 29 10759.0 30 12592.0 31 15271.0 32 19405.0 33 25997.0 34 37789.0 35 42847.0 36 14920.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 48.86256950964108 17.01111729446599 7.46392295634221 26.66239023955072 2 28.36661002548853 20.865335598980458 26.38912489379779 24.37892948173322 3 27.854408897234055 20.576128190858924 23.65558052498099 27.913882386926026 4 24.32412914188615 15.498725573491928 24.63806287170773 35.53908241291419 5 25.48555649957519 27.247663551401867 27.024214103653353 20.242565845369583 6 41.19498725573492 25.99915038232795 14.445199660152932 18.360662701784197 7 36.36834793986329 24.873723964196657 15.33324553838834 23.42468255755171 8 33.9927782497876 27.99362786745964 16.713678844519965 21.299915038232793 9 30.253186066270178 15.387425658453695 15.409090909090908 38.95029736618522 10-11 35.83631231162343 19.983474901178635 19.750891569438338 24.429321217759593 12-13 33.63806287170773 19.836873406966866 19.368521665250636 27.156542056074766 14-15 33.211129991503824 18.61682242990654 17.141036533559898 31.03101104502974 16-17 34.06520815632965 21.33283772302464 17.267204757858963 27.33474936278675 18-19 32.98003398470688 21.805437553101104 18.319031435853866 26.89549702633815 20-21 33.689889549702634 21.09558198810535 18.056924384027187 27.157604078164827 22-23 34.07094307561597 20.912064570943077 17.465802888700086 27.551189464740865 24-25 34.07519116397621 20.55947323704333 17.322429906542055 28.042905692438403 26-27 33.57228881114871 20.247918963294314 18.72242719292097 27.457365032636012 28-29 33.840271877655056 20.576677994902294 18.95836873406967 26.624681393372985 30-31 33.887213254035686 20.630628717077315 18.255734919286322 27.226423109600677 32-33 33.217714528462196 21.276125743415463 18.757221750212405 26.748937977909943 34-35 33.63070204504711 20.62570625069032 18.791153705638962 26.95243799862361 36-37 33.58673234225221 20.045709527845215 19.08776356789203 27.27979456201054 38-39 32.734703067351745 20.241845211418084 20.34656125676247 26.6768904644677 40-41 32.85761621757102 19.545749981945548 20.64770878380962 26.94892501667382 42-43 32.240866610025485 19.293118096856414 21.194350042480885 27.271665250637213 44-45 31.16864910790144 20.16376380628717 21.75233644859813 26.915250637213255 46-47 31.050764655904846 20.436703483432456 21.50467289719626 27.00785896346644 48-49 30.641886151231944 19.691376380628718 22.599405267629567 27.06733220050977 50-51 29.818819031435854 20.166525063721323 22.59961767204758 27.41503823279524 52-53 29.274001699235342 21.709855564995753 22.101316907391674 26.914825828377232 54-55 29.633177570093455 20.95964316057774 21.65802888700085 27.749150382327954 56-57 29.14401019541206 21.689889549702634 21.46304163126593 27.70305862361937 58-59 29.54226847918437 21.62213254035684 21.685641461342396 27.1499575191164 60-61 29.47875955819881 21.244052676295667 20.85089209855565 28.426295666949876 62-63 29.409952994203397 20.814030794850392 21.43659740055608 28.33941881039013 64-65 29.909940526762956 20.562234494477487 21.140824129141887 28.38700084961767 66-67 30.251274426508072 20.2446898895497 20.05395072217502 29.450084961767203 68-69 30.042056074766354 20.08836023789295 20.23088360237893 29.638700084961766 70-71 30.00169923534409 19.807136788445202 20.205395072217502 29.985768903993204 72-73 29.76781925118469 18.81405361546484 20.496307325662652 30.921819807687818 74-75 29.716864910790143 18.95836873406967 21.006159728122345 30.318606627017843 76 30.119371282922685 18.79226847918437 20.69881053525913 30.38954970263382 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.5 3 2.5 4 4.0 5 3.0 6 2.0 7 3.5 8 5.0 9 7.0 10 10.0 11 14.5 12 18.0 13 22.5 14 30.5 15 45.0 16 69.5 17 83.0 18 113.0 19 155.0 20 189.0 21 211.0 22 272.0 23 351.5 24 462.5 25 555.0 26 617.0 27 757.5 28 988.0 29 1140.0 30 1283.0 31 1603.0 32 2033.0 33 2286.0 34 2421.5 35 2752.0 36 3249.0 37 3551.0 38 3807.5 39 4285.5 40 4622.5 41 4886.5 42 5035.0 43 5153.5 44 5494.0 45 5718.0 46 5720.0 47 5746.5 48 5776.5 49 5860.0 50 5940.0 51 5989.0 52 6173.5 53 6535.5 54 6762.0 55 7027.5 56 7485.0 57 7901.0 58 8125.0 59 8166.0 60 8371.5 61 8567.0 62 8598.0 63 8525.0 64 8348.5 65 8007.0 66 7672.0 67 7575.0 68 7297.5 69 6760.5 70 6218.5 71 5936.0 72 5617.5 73 5002.0 74 4381.5 75 4058.0 76 3736.0 77 3142.5 78 2563.5 79 2256.0 80 2020.0 81 1566.5 82 1182.5 83 1016.0 84 854.0 85 577.5 86 396.0 87 329.0 88 294.0 89 238.5 90 213.5 91 207.0 92 205.0 93 240.0 94 294.0 95 380.5 96 448.0 97 552.0 98 940.0 99 6880.0 100 12536.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 4.248088360237893E-4 2 0.0 3 4.248088360237893E-4 4 0.0 5 0.0 6 0.0 7 0.001274426508071368 8 0.0 9 0.0 10-11 2.1240441801189465E-4 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 2.1240441801189465E-4 28-29 0.0 30-31 0.0 32-33 0.0 34-35 8.496176720475786E-4 36-37 2.1240441801189465E-4 38-39 6.37213254035684E-4 40-41 4.248088360237893E-4 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0014868309260832626 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 6.37213254035684E-4 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 235400.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 68.04342372518003 #Duplication Level Percentage of deduplicated Percentage of total 1 77.93525322317211 53.02981458193502 2 12.125188911039277 16.500787336433994 3 5.785482631969119 11.809921385452336 4 2.540539619231378 6.9146805520787264 5 0.9600442717854788 3.266234959001561 6 0.36199477989931705 1.4778818516995509 7 0.15190323285088808 0.7235211226668364 8 0.06103130616969889 0.3322223220964812 9 0.027662811187016623 0.16940451447250401 >10 0.048385939426352366 0.5113631646873751 >50 0.0012566366346824724 0.0688322164968384 >100 6.283183173412362E-4 0.06070915989162145 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 6.283183173412362E-4 5.134626833087138 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 12010 5.1019541206457095 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.028462192013593884 0.0 2 0.0 0.0 0.0 0.031011045029736617 0.0 3 0.0 0.0 0.0 0.03228547153780799 0.0 4 0.0 0.0 0.0 0.0437553101104503 0.0 5 0.0 0.0 0.0 0.045879354290569246 0.0 6 0.0 0.0 0.002548853016142736 0.04927782497875956 0.0 7 0.0 0.0 0.002548853016142736 0.05310110450297366 0.0 8 0.0 0.0 0.002548853016142736 0.05395072217502124 0.0 9 0.0 0.0 0.002548853016142736 0.06032285471537808 0.0 10 0.0 0.0 0.002548853016142736 0.062446898895497024 0.0 11 0.0 0.0 0.002548853016142736 0.06711979609175871 0.0 12 0.0 0.0 0.002548853016142736 0.06924384027187766 0.0 13 0.0 0.0 0.002548853016142736 0.07476635514018691 0.0 14 0.0 0.0 0.002548853016142736 0.07858963466440103 0.0 15 0.0 0.0 0.002548853016142736 0.08071367884451996 0.0 16 0.0 0.0 0.002548853016142736 0.0875106202209006 0.0 17 0.0 0.0 0.002548853016142736 0.08920985556499575 0.0 18 0.0 0.0 0.002548853016142736 0.09388275276125743 0.0 19 0.0 0.0 0.002548853016142736 0.09473237043330501 0.0 20 0.0 0.0 0.002548853016142736 0.09855564995751911 0.0 21 0.0 0.0 0.002548853016142736 0.10195412064570943 0.0 22 0.0 0.0 0.002548853016142736 0.11257434154630416 0.0 23 0.0 0.0 0.002548853016142736 0.11554800339847068 0.0 24 0.0 0.0 0.002548853016142736 0.12446898895497026 0.0 25 0.0 0.0 0.002548853016142736 0.1265930331350892 0.0 26 0.0 0.0 0.002548853016142736 0.12999150382327954 0.0 27 0.0 0.0 0.002548853016142736 0.17162276975361088 0.0 28 0.0 0.0 0.002548853016142736 0.22047578589634664 0.0 29 0.0 0.0 0.002548853016142736 0.2931180968564146 0.0 30 0.0 0.0 0.002548853016142736 0.3615123194562447 0.0 31 0.0 0.0 0.002548853016142736 0.443500424808836 0.0 32 0.0 0.0 0.002548853016142736 0.5556499575191164 0.0 33 0.0 0.0 0.002548853016142736 0.7149532710280374 0.0 34 0.0 0.0 0.002548853016142736 0.975785896346644 0.0 35 0.0 0.0 0.002548853016142736 1.3237043330501275 0.0 36 0.0 0.0 0.002548853016142736 1.85089209855565 0.0 37 0.0 0.0 0.002548853016142736 2.568819031435854 0.0 38 0.0 0.0 0.002548853016142736 3.36321155480034 0.0 39 0.0 0.0 0.002548853016142736 4.162701784197111 0.0 40 0.0 0.0 0.002548853016142736 5.077740016992354 0.0 41 0.0 0.0 0.002548853016142736 5.911214953271028 0.0 42 0.0 0.0 0.002548853016142736 6.821580288870009 0.0 43 0.0 0.0 0.002548853016142736 7.7527612574341545 0.0 44 0.0 0.0 0.002548853016142736 8.796516567544606 0.0 45 0.0 0.0 0.002548853016142736 9.725998300764656 0.0 46 0.0 0.0 0.002548853016142736 10.713678844519967 0.0 47 0.0 0.0 0.002548853016142736 11.715802888700084 0.0 48 0.0 0.0 0.002548853016142736 12.728547153780799 0.0 49 0.0 0.0 0.002548853016142736 13.613848768054375 0.0 50 0.0 0.0 0.002548853016142736 14.476635514018692 0.0 51 0.0 0.0 0.002548853016142736 15.305437553101104 0.0 52 0.0 0.0 0.002548853016142736 16.048853016142736 0.0 53 0.0 0.0 0.002548853016142736 16.753186066270178 0.0 54 0.0 0.0 0.002548853016142736 17.44604927782498 0.0 55 0.0 0.0 0.002548853016142736 18.247663551401867 0.0 56 0.0 0.0 0.002548853016142736 19.05140186915888 0.0 57 0.0 0.0 0.002548853016142736 19.881478334749364 0.0 58 0.0 0.0 0.002548853016142736 20.615972812234496 0.0 59 0.0 0.0 0.002548853016142736 21.344519966015294 0.0 60 0.0 0.0 0.002548853016142736 22.058623619371282 0.0 61 0.0 0.0 0.002548853016142736 22.750637213254034 0.0 62 0.0 0.0 0.002548853016142736 23.417587085811384 0.0 63 0.0 0.0 0.002548853016142736 24.086661002548855 0.0 64 0.0 0.0 0.002548853016142736 24.714528462192014 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TGTATCG 15 0.0022227007 70.00001 5 AATTTAC 20 0.0069452054 52.5 67 TACGGTA 20 0.0069452054 52.5 56 TACGTAG 20 0.0069452054 52.5 46 GGGTTAT 30 5.869827E-4 46.666668 1 TTTACTA 35 0.0012537357 40.000004 2 TTCATAC 55 2.98628E-4 31.81818 52 TTTAGCA 45 0.0043015718 31.111113 18 TACTACA 45 0.0043015718 31.111113 2 GGTAGAC 45 0.0043015718 31.111113 45 GCTTTCT 45 0.0043015718 31.111113 1 AAGACGG 60 4.972402E-4 29.166668 5 AAAGGTC 60 4.972402E-4 29.166668 23 AGCAATC 50 0.007198909 27.999998 5 TTAGCAT 50 0.007198909 27.999998 19 CGACTCA 50 0.007198909 27.999998 70 CCAGTTC 50 0.007198909 27.999998 13 TTGGGCA 50 0.007198909 27.999998 53 ATGTAAA 65 7.9404464E-4 26.923077 39 ACAGACG 65 7.9404464E-4 26.923077 66 >>END_MODULE