FastQCFastQC Report
Wed 25 May 2016
SRR1779644_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779644_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences235400
Sequences flagged as poor quality0
Sequence length76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGT64652.7463891248937977No Hit
TCTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCG16280.6915887850467289No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCC4790.20348343245539507No Hit
TCTTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCC2640.11214953271028037No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCATAA150.002224681269.9842829
AGGGGGG5600.054.36279370
CTATCGG200.00694553952.4993640
CAGTAAA200.00694553952.499361
TAGCGAG200.006951377352.4882128
CTAGTGA200.006951377352.4882129
CTTTATA651.8553692E-1048.4506572
CTACTGC451.677723E-646.67601851
CGTCTTA305.864029E-446.67601849
TCGTATG14850.045.72335440
CGTATGC14450.045.5745141
TATGCCG14150.045.30296743
GTATGCC13950.045.1991542
CGTATGA703.783498E-1045.03772741
CTCGTAT14850.044.780639
TCTCGTA15000.044.33279838
TTGAAAA14400.044.0018758
TAGATCT14100.043.92630434
ATCGTAT404.5839515E-543.74946639
TTTATAC802.910383E-1143.7401733
Produced by FastQC (version 0.11.5)