FastQCFastQC Report
Wed 25 May 2016
SRR1779643_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779643_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences233590
Sequences flagged as poor quality0
Sequence length76
%GC65

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG130395.582002654223212No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCTAGG150.00222267970.045
TGATTCT150.00222267970.06
TGCAGTA200.00694513952.514
TAAACCA305.8697327E-446.6666645
GTGGTAC305.8697327E-446.6666641
CTGTAAC350.001253715740.048
ATATTAT400.00241550235.045
AATTTCA552.986209E-431.8181820
TAAACCT552.986209E-431.818185
AACCGGT450.00430150331.11111312
CTGGACT450.00430150331.1111134
TTGGGGG450.00430150331.1111132
ATAAACC604.9722847E-429.1666664
TGGACTG853.9412607E-628.823535
AGCCATA500.007198793328.00000212
GTATAAG500.007198793328.0000021
GCTTGGA657.94026E-426.9230772
ATAAGAG700.001223629825.03
CTTGGAG800.002660850821.8753
TAAGAGA800.002660850821.8754
Produced by FastQC (version 0.11.5)