FastQCFastQC Report
Wed 25 May 2016
SRR1779643_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779643_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences233590
Sequences flagged as poor quality0
Sequence length76
%GC63

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGT71533.062203005265636No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCG15410.6597028982405069No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCC7480.3202191874652168No Hit
ATTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGT2550.10916563209041484No Hit
TCTTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCC2470.10574082794640183No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAT150.002219066770.028741
CTAGAAT150.002224729669.9837339
ATAGGAC207.924536E-569.983733
CTTTATA301.022363E-769.983732
TGTGTCC150.002224729669.983738
TCTGCTA453.8198777E-1062.26107853
TTTATAC401.0641088E-861.2357643
AGGGGGG6150.056.89734370
TATCTCT252.3772064E-456.04697845
TACTATC252.3772064E-456.04697842
TAGAAAA750.055.9989758
CGTATGA653.6379788E-1253.8682441
CTAGAAA800.052.49903557
AGTACTA200.00694564152.49903540
AAATAGG200.00695152652.4877971
CGTTAAA407.470444E-752.48779728
GTTAAAA407.470444E-752.48779729
TCTTTAT407.470444E-752.4877971
CAGAGGT200.00695152652.4877974
GCTAGAA750.051.34338856