FastQCFastQC Report
Wed 25 May 2016
SRR1779642_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779642_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences449251
Sequences flagged as poor quality0
Sequence length76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG120032.6717803633158304No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGGTC459.192953E-538.88888540
GTATGTA601.2149005E-535.01
GGGTAGT450.00430569631.11110936
TCCAAGT450.00430569631.11110924
GCACTTA450.00430569631.1111091
CGCTTAG450.00430569631.11110932
ACTGGAC450.00430569631.1111093
TCTAAGC802.4641904E-630.6253
TTCTAAG604.979518E-429.1666662
TAAAACG500.00720576927.99999829
TAAGACG657.951749E-426.92307753
AGTTGCG700.001225391625.05
GGTGCAA700.001225391625.022
GTTATAG750.00183130123.3333342
AACTTAT1202.1640517E-623.33333245
GTGCAAC850.003787223520.58823623
CATCGTA850.003787223520.58823624
TTAGGCC1257.812829E-519.64
GCTCTAA900.005271962419.4444431
TCTATAC1107.467431E-419.0909083
Produced by FastQC (version 0.11.5)