FastQCFastQC Report
Wed 25 May 2016
SRR1779642_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779642_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences449251
Sequences flagged as poor quality0
Sequence length76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGT62421.3894237297190213No Hit
TCTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCG15970.3554805665429793No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCC5810.1293263676652918No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATTACG200.006954321652.4904022
TACCGAT200.006954321652.4904029
TTTACGA305.851873E-446.70491846
AGGGGGG6350.045.73966670
TCGTATG13350.043.5126440
CTCGTAT12650.043.43079439
CGTATGC12750.043.3984341
GTATGCC12950.042.73770542
CTTTATA757.566996E-1041.992322
TCTCGTA12650.041.7710238
CCGTCTT12650.041.5359947
CTTGAAA13000.041.46780457
TCTTCTG12750.041.19186450
TTGAAAA13600.040.925358
GTCTTCT12850.040.87130749
TATGCCG13900.040.57279643
TGAAAAA14850.039.8376759
GAAAAAA15800.039.64464660
ATGCCGT14150.039.6084144
GCCGTCT13550.039.5526946
Produced by FastQC (version 0.11.5)