FastQCFastQC Report
Wed 25 May 2016
SRR1779641_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779641_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences104095
Sequences flagged as poor quality0
Sequence length76
%GC74

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1966318.889475959460107No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2000.1921321869446179No Hit
CGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1940.18636822133627937No Hit
GGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1170.11239732936260147No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCTTTC150.002216666770.02019567
GATTTTG150.002216666770.02019534
CTAAGAT150.002216666770.02019530
TCTAAGA150.002216666770.02019529
TGCCTTT150.002216666770.02019566
GTCCTCT150.00222089969.9865425
ATTTTGT150.00222089969.9865435
TCTAATC150.00222089969.9865447
TTTTGTA150.00222089969.9865436
CTTTAGT150.00222089969.986541
TAAGCAT150.00222089969.9865441
TGTAAGC150.00222089969.9865439
CCTCTAA150.00222089969.9865427
TAATCAC252.3831714E-455.9892449
TGAACTC200.006926455552.5151445
ACAATAC200.006926455552.51514460
AGATTTT200.006926455552.51514433
TAAATGC200.006926455552.51514462
TAAGATT200.006926455552.51514431
CTCTAAG200.006926455552.51514428
Produced by FastQC (version 0.11.5)