FastQCFastQC Report
Wed 25 May 2016
SRR1779641_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779641_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences104095
Sequences flagged as poor quality0
Sequence length76
%GC61

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGT1069410.273308035928718No Hit
TCTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCG25312.4314328257841393No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCC9970.9577789519189202No Hit
TCTTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCC4240.40732023632258996No Hit
ATTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGT3420.3285460396752966No Hit
ATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGTCTT3340.3208607521975119RNA PCR Primer, Index 15 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCAGT2590.24881118209328015No Hit
CTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGACGT2570.24688986022383402No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2520.24208655555021855No Hit
CTTATACACATCTCCGAGCCCACGAGACATAAGTTAATATCGTATGCCGT2470.23728325087660312No Hit
CTTATACACATCTCCGAGCCCACGAGACATAAGATAATCTCGTATGCCGT2310.22191267592103367No Hit
CTTATACACATCTCCGAGCCCACGAGACATAAGTAAATCTCGTATGCCGT1840.17676161198904847No Hit
CTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGTC1310.1258465824487247No Hit
CTTATACACATCTCCGAGCCCACGAGACATAAGTTATCTCGTATGCCGTC1110.10663336375426294No Hit
CTTATACACATCTCCGAGACCACGAGACATAAGTTAATCTCGTATGCCGT1070.10279072001537057No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAATGCA150.002209121870.0803742
AATGCAG150.002209121870.0803743
CACGTAT207.8875644E-570.01298539
CTACTTG150.00221757270.0129854
TACTGAT150.00221757270.0129852
ACTAATG150.00221757270.0129850
ACGTATG150.00221757270.0129840
GACTAAT150.00221757270.0129849
TAAAACT150.002221806269.9793334
CACTGAC150.002221806269.979339
GTTAAAA150.002221806269.9793333
AAACACG150.002221806269.9793336
TATAAGC150.002221806269.9793331
CTTTATA900.066.091592
TCTTTAT850.065.862911
AATATCG1000.062.98140736
TTTATAC1000.062.9814073
GTTAAAC453.8016879E-1062.2038533
TAAACTC401.0570147E-861.23192235
AGGGGGG8000.060.3571870
Produced by FastQC (version 0.11.5)