FastQCFastQC Report
Wed 25 May 2016
SRR1779640_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779640_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences189412
Sequences flagged as poor quality0
Sequence length76
%GC60

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG136637.21337613245201No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTGA150.002222028870.01
TTACTCG150.002222028870.04
TAAGACT308.353212E-658.3333324
TAAAACG200.00694312152.54
GTATGCG200.00694312152.52
ACTGTTA200.00694312152.570
TTTCAGG350.001253115540.030
CTTACCG350.001253115540.09
TTAGGAA501.7051051E-435.0000044
GTATTAC552.98409E-431.8181841
TCGATAC450.004299469331.11110959
CATTAGG450.004299469331.1111091
GTGTGTC604.9687776E-429.16666611
AGACGAG500.00719541128.0000027
TCTCCCA500.00719541128.0000024
AAGGGAC500.00719541128.00000238
TGAGAGA500.00719541128.00000251
GATAGAA500.00719541128.0000027
TAATAGA657.934683E-426.9230774
TATATAA657.934683E-426.92307719
Produced by FastQC (version 0.11.5)