FastQCFastQC Report
Wed 25 May 2016
SRR1779640_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779640_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences189412
Sequences flagged as poor quality0
Sequence length76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGT79264.184528963318058No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCG18160.9587565729732012No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCC6350.33524803074778786No Hit
TCTTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCC2490.13145946402551054No Hit
ATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGTCTT2240.11826072265748738Illumina PCR Primer Index 9 (95% over 21bp)
ATTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGT2220.11720482334804552No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTTAT252.3780593E-456.0374150
AGGGGGG6200.055.31569370
CTTTATA452.6901034E-854.437672
CGTCTAA200.006924852.53507248
TTAATAC200.006946538652.4934653
AATGCCG352.0758484E-550.02018743
TATGCCG14500.049.98568743
GTATGCC14600.049.8831442
CGTATGC14950.049.8731841
TCGTATG15500.049.67123840
ATGCCGT14550.049.57327344
CCGTCTT13400.049.3986547
CTCGTAT15600.049.12849839
GCCGTCT13550.049.11027546
CTGCTTG14150.048.98209854
CTTGAAA14650.048.97001357
TCTCGTA15700.048.8155838
TCTGCTT14450.048.70477353
CGTCTTC14000.048.532448
TTGAAAA15400.048.1758258
Produced by FastQC (version 0.11.5)