FastQCFastQC Report
Wed 25 May 2016
SRR1779639_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779639_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences425185
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG106162.4967955125416No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTAGC252.3893185E-455.9999961
GCGTAAG200.0069490452.531
TGCTTAA350.001254876540.038
ATACTGG1001.256376E-831.4999986
CTGGACA1101.0143831E-625.4545444
TGGCGTA750.0018311423.33333424
CCGCATT1052.0367113E-523.33333452
TAACTAC1102.9178707E-522.27272643
GCTTTAA1102.9178707E-522.2727261
GTACATG800.002664410521.8751
TACTCAC800.002664410521.8752
TCCTTAC6750.021.25925870
ATAGATC1003.928011E-421.03
GACTCAG1003.928011E-421.029
AGACTTC850.003786894520.58823611
GTTTATC850.003786894520.5882362
CGTTTAG850.003786894520.58823627
TTCGAGT850.003786894520.58823622
ATCGAAT850.003786894520.58823622
GCATATT850.003786894520.58823621
Produced by FastQC (version 0.11.5)