FastQCFastQC Report
Wed 25 May 2016
SRR1779639_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779639_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences425185
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGT40340.9487634794266027No Hit
TCTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCG10830.25471265449157426No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGTAT452.701745E-854.43548239
CGTACGT200.00695356552.491362
CGTATGA950.051.60079641
TCGTATG8300.048.48599240
CTCGTAT8200.047.7970139
TCTCGTA8500.047.34514238
CGTATGC7700.046.83801741
CGTCTTA1650.046.70292348
TGTAACG305.8799726E-446.6589833
ATAGAAC404.5943605E-543.7427983
GTATGCC8950.043.4364342
ATCTCGT9300.043.27244637
CCGTCTT6600.042.45720347
GTATGAC1500.042.02768342
TATGCCG9250.040.89180443
GTCTTCT5250.040.68389549
CTTGAAA7850.040.57134257
TGCCGTA1400.040.02636745
GTCTTAA350.00125226540.01694549
CGGATAT701.9261279E-839.99341634