FastQCFastQC Report
Wed 25 May 2016
SRR1779638_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779638_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences490361
Sequences flagged as poor quality0
Sequence length76
%GC60

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG173843.545143271997569No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACATT350.001255064740.04
AACTTAT652.1015761E-532.30769345
AACATTG450.004306075631.11111323
CGTATAC500.007206401327.99999864
TACTTGT500.007206401327.9999986
CTATAGA500.007206401327.9999981
AGACAGT500.007206401327.9999981
GTATCAT906.1661012E-627.22222357
CGTATCA1101.0149142E-625.45454856
ATATACT851.307525E-424.7058834
CTTACGT6850.024.52554770
AGTAGTG750.001831538723.3333345
CGTGTGG1202.1647265E-623.3333329
TACACCG1052.0375384E-523.3333325
GTACACG800.00266498821.8754
TGTACCT800.00266498821.87542
GCGTGTG1304.336518E-621.5384628
CAGCTAA850.00378771120.5882361
TATTAAA1558.728239E-720.3225848
CTCCTTA18350.020.02724870
Produced by FastQC (version 0.11.5)