FastQCFastQC Report
Wed 25 May 2016
SRR1779638_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779638_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences490361
Sequences flagged as poor quality0
Sequence length76
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGT96301.9638592791841114No Hit
TCTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCG21560.43967607538119874No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCC6630.1352065111213983No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGA1800.056.41009541
CCGTTTA252.3912638E-455.99249340
GTATTCT608.367351E-1152.5143849
TATACGT200.00695335652.492965
CGTCTTA750.051.36311348
GTATGAC1800.050.57972742
TCGTATG20150.050.5391340
AGGGGGG7800.049.80101870
CTCGTAT20300.049.64851839
CTTGAAA18350.049.21325357
TGAAAAA21800.048.97131359
GTATGCC19100.048.76675442
GTCTTCT16400.048.67186749
CCGTCTT16450.048.53878447
CTTTATA651.8553692E-1048.4550442
TATCGTA651.8553692E-1048.45504438
TTGAAAA19400.048.35389758
CGTATGC19300.048.25647741
TCTCGTA21800.047.35603738
TCTTCTG16750.047.0278150
Produced by FastQC (version 0.11.5)