FastQCFastQC Report
Wed 25 May 2016
SRR1779637_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779637_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences411112
Sequences flagged as poor quality0
Sequence length76
%GC61

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG141363.438479051937185No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACAAGT350.001254978139.99902724
GTAGTTA400.002416478235.0034071
AGTATAC400.002417920634.9991539
GTATACT400.002417920634.9991540
GTACTGC652.0993562E-532.3108376
GTATAAT552.988429E-431.8212761
CACCTAG500.00720164328.0027287
TAAATGC500.00720164328.0027284
CACAAGT500.00720164328.0027287
AAGTATA500.00720592127.99932138
TTACAAG657.9517235E-426.92242223
GTGTAGC1056.7531255E-726.6692621
CCATACG700.00122538824.99939270
ACAAGTA700.00122538824.99939225
TAGAACG851.3061591E-424.7082864
GTCCTAC901.9246715E-423.3356041
CTTTTTC750.001831297323.33276622
GGAAGTT750.001831297323.33276619
TCCTTAC10200.021.9602570
CCTTACG7050.021.34699870
Produced by FastQC (version 0.11.5)