FastQCFastQC Report
Wed 25 May 2016
SRR1779637_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779637_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences411112
Sequences flagged as poor quality0
Sequence length76
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGT78061.8987526513456188No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCG17420.423728813559322No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCC7320.17805366907314796No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATAAG150.002225218869.9894226
TTAATTG200.006953032752.49206516
AGGGGGG6950.051.86266370
ACTCGTA352.0839787E-550.00460438
CGTATGC15900.049.5449241
GTATGCC15650.049.44751742
CCGTCTT14600.048.92646847
TTGAAAA15400.048.4194458
TCGTATG16900.047.84464640
GTCTTCT14700.047.61763849
CTTGAAA15750.047.1211857
TCTTCTG15000.046.89861350
CTCGTAT16950.046.67096739
TAGCATA451.6848862E-646.6596133
TCTTTAT751.4551915E-1146.659611
GCCGTCT15400.046.61221746
TCTGCTT15050.046.52723353
TGAAAAA17750.045.95348459
CTTCTGC15100.045.89268551
TATGCCG16800.045.8542943
Produced by FastQC (version 0.11.5)