FastQCFastQC Report
Wed 25 May 2016
SRR1779636_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779636_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences239279
Sequences flagged as poor quality0
Sequence length76
%GC66

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG174297.2839655799297045No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTGTAC150.002222746170.049
TATTACG150.002222746170.060
CTTTAAC150.002222746170.041
GTGTTTT200.006945344652.542
TTGTGCC200.006945344652.541
TTGTACC200.006945344652.550
TAACGAG200.006945344652.518
TTATAAC200.006945344652.52
AGGGGTT200.006945344652.548
TAGTGGA200.006945344652.54
AATAACC200.006945344652.523
ACTATAT350.001253777240.042
ATCACAG350.001253777240.065
CTCTGGA350.001253777240.02
CTCTAAA350.001253777240.049
TTGTGCG501.7064458E-435.02
AACTTAT501.7064458E-435.045
TTATGCC400.002415619335.04
TCCCATC400.002415619335.08
GGGACTC450.004301711531.11110952
Produced by FastQC (version 0.11.5)