FastQCFastQC Report
Wed 25 May 2016
SRR1779636_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779636_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences239279
Sequences flagged as poor quality0
Sequence length76
%GC62

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGT93243.896706355342508No Hit
TCTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCG22840.9545342466325921No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCC8800.3677715135887395No Hit
TCTTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCC3460.14460107238829983No Hit
ATTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGT3120.13039171845418948No Hit
ATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGTCTT2980.12454080801073224TruSeq Adapter, Index 12 (95% over 23bp)
CTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGACGT2740.11451067582194843No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAGTA207.915926E-569.9997938
AATCTAG207.915926E-569.9997936
CACTGAG150.002224615869.9851664
CTTTGTA150.002224615869.985162
ACTAATT207.9241276E-569.9851627
AATGTCT150.002224615869.9851622
TCTTTAT550.063.622871
AGGGGGG6950.060.92233370
CTAGTAT308.360055E-658.33315339
ACGTATG308.360055E-658.33315340
TAGTATG308.360055E-658.33315340
CTTTATA601.8189894E-1258.320972
TTATGCT252.3823045E-456.02325452
AAATCTA252.3897007E-455.98813235
AGTATGC452.687193E-854.4556641
TTTATAC653.6379788E-1253.834743
CGACTTA200.006916755352.5547748
TCTGCTA200.006928212552.53278453
TTTACGT200.006933946652.521851
AACAAAG200.006951169652.48887366
Produced by FastQC (version 0.11.5)