FastQCFastQC Report
Wed 25 May 2016
SRR1779635_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779635_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences768574
Sequences flagged as poor quality0
Sequence length76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG170712.221126397718372No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAATCG450.00430758231.1111134
ACGTTAG500.007208904728.00000270
GTCTTAA700.001226184825.01
CTCCTTA23200.023.53448370
GTATTAA952.7802453E-422.1052631
CGATTAG952.7802453E-422.10526319
TCCTTAC14450.021.07266470
TTCGGAC850.00378963820.5882363
GCCTAAG1408.245408E-620.01
TATACCG1257.822055E-519.66
ACTCCTT27200.018.65808969
TTGTACC950.00720848418.4210514
AAACCGT1200.001340520117.5000026
TGCACAC1401.8646622E-417.53
CCTTACG9650.017.04663370
AACTTAT1452.4379989E-416.89655145
CTGTACG1250.001761876716.81
GTATAGA1250.001761876716.81
ACTGTCC1503.1574327E-416.3333348
CTAACTA1554.0529648E-415.80645342
Produced by FastQC (version 0.11.5)