FastQCFastQC Report
Wed 25 May 2016
SRR1779635_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779635_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences768574
Sequences flagged as poor quality0
Sequence length76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGT82371.07172503883816TruSeq Adapter, Index 5 (95% over 23bp)
TCTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCG18580.2417464030789488TruSeq Adapter, Index 5 (95% over 22bp)
CTCTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCC8070.10499964870006012RNA PCR Primer, Index 5 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC16950.047.919141
TCGTATG16650.047.50543640
GTATGCC16750.047.45855742
CTCGTAT16200.046.66462739
CCGTCTT14750.046.31134847
TATGCCG17150.045.53784643
CTTGAAA17100.044.00392557
TCTCGTA17450.043.92072338
TATACCG404.598093E-543.7423976
AGGGGGG8550.043.3842770
CGTCTTC15100.042.9180248
GCCGTCT15950.042.82711446
GTCTTCT14950.041.46059449
TTGAAAA18500.041.2414458
ATGCCGT19250.041.11854644
TCTTCTG15250.040.64497850
CTGCTTG16150.040.5403754
TGAAAAA21750.040.3890459
TCTGCTT16200.040.20435753
GCTTGAA18400.040.13934356
Produced by FastQC (version 0.11.5)