FastQCFastQC Report
Wed 25 May 2016
SRR1779634_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779634_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences477863
Sequences flagged as poor quality0
Sequence length76
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG181173.7912539786507846No Hit
CTTATACACATCTGACGCTGCCGACGATCTACTCTGTGTAGATCTCGGTG7610.15925066389320788No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCCAA604.9799867E-429.16666811
CTAGATG500.0072062227.99999837
GTTTAGC500.0072062227.9999981
TGCTAAC500.0072062227.9999982
TTAGAGT657.952494E-426.9230774
TGTATAA657.952494E-426.92307711
GTGTATA657.952494E-426.92307710
TACTGGC700.00122550625.07
CTCTGGT851.3074566E-424.7058831
ATCTATT1001.39653985E-524.49999852
TTTTCGC750.001831471323.33333449
AGTGTGC750.001831471323.33333410
TCCAACG1102.9188475E-522.2727282
TAGAGTT1003.928993E-421.05
GTATAAG1506.3692823E-721.01
AGGTTTA850.00378757120.58823617
TGCACTT850.00378757120.58823630
TATGCTT1055.4601417E-420.00000210
AGTACAA1055.4601417E-420.0000025
GTTCTAC1257.8141544E-519.61
Produced by FastQC (version 0.11.5)