FastQCFastQC Report
Wed 25 May 2016
SRR1779634_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779634_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences477863
Sequences flagged as poor quality0
Sequence length76
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGT106362.2257425245310896No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCG18960.3967664372424733No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG13310.2785317130642046No Hit
ATTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGT10850.22705252342198495No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCC10370.21700780349179577No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG13000.054.63652470
CTTGAAA21650.047.05851757
CTCGTAT21550.046.93230439
TCGTATG22200.046.82908640
TCTCGTA21800.046.71515338
CGTATGC21850.046.47727241
CCGTCTT21200.046.44484347
GTATGCC21550.046.3312142
TCTTCTG21850.046.3218650
TTGAAAA22400.046.2595558
GTCTTCT21900.046.21610349
AATCTCG22350.045.4089836
TGAAAAA23750.044.95664659
TGCTTGA22400.044.87649555
TATGCCG22300.044.61588743
TCTGCTT22700.044.44236453
GCCGTCT22350.044.0550746
GAAAAAA24800.043.89559660
CTGCTTG22750.043.72421354
CGTCTTC22950.043.66670248