FastQCFastQC Report
Wed 25 May 2016
SRR1779633_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779633_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences444432
Sequences flagged as poor quality0
Sequence length76
%GC63

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG175783.955160744500846No Hit
CTTATACACATCTGACGCTGCCGACGATCTACTCTGTGTAGATCTCGGTG6800.15300428411995534No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTGTG150.002224004170.05
GGTGTAT200.00694924652.51
TGCTACA200.00694924652.52
GCGTATG200.00694924652.559
CCATTAA200.00694924652.57
ATCATTA1450.043.44827759
TCATTAA1500.042.00000460
ATGTAAA350.00125493840.039
TGTAAAA350.00125493840.040
TATCATT1600.039.37558
CATTAAA1600.039.37561
TTGATAC556.6899374E-638.181823
ATTAAAA1800.036.94444362
ACTTGCT400.002417842235.063
CGTATCA1800.034.99999656
GTATCAT1800.034.99999657
TAGATCT1950.032.30769339
GAATCCT552.990522E-431.81818436
TGTATAA450.004305646331.1111092
GTGCTAC450.004305646331.1111091
Produced by FastQC (version 0.11.5)