FastQCFastQC Report
Wed 25 May 2016
SRR1779633_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779633_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences444432
Sequences flagged as poor quality0
Sequence length76
%GC62

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGT99462.2379126615545237No Hit
TCTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCG18660.41986175612917165No Hit
ATTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGT12930.2909331461280916No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG12390.27878280591856575No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCC11070.24908197429528026No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTATT150.002213484870.0837246
TATCTAG207.933816E-569.9812136
TCTTAAT308.385003E-658.3176731
AGGGGGG9700.057.7164670
TAGTATG252.3872906E-456.0101840
ATTATAC2500.055.9849661
GTCTTAT653.6379788E-1253.8801949
GTATCTA200.00695662752.48590535
CGTAACT200.00695662752.48590534
TCTTTAT407.4877244E-752.4859051
CTTTATA407.4877244E-752.4859052
CTAGTAT352.085925E-549.9978339
TCTAGTA352.0873142E-549.99220338
TATGCTT506.153823E-849.03650353
TTATGCT604.9021764E-946.6961752
TTTATAC451.6865579E-646.654143
TAACTCG305.8832695E-446.65413736
TCGTATG20050.046.6169540
GAAAAAA21900.046.339860
CGTATGC19850.046.2152741
Produced by FastQC (version 0.11.5)