FastQCFastQC Report
Wed 25 May 2016
SRR1779632_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779632_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences192188
Sequences flagged as poor quality0
Sequence length76
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG152447.931816762753137No Hit
CTTATACACATCTGACGCTGCCGACGATCTACTCTGTGTAGATCTCGGTG5750.29918621349928193No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCGTT150.002222078270.07
CCGTATC1300.053.84615355
GCCGTAT1300.053.84615354
AACCGTG200.00694327452.523
GTACCGT200.00694327452.56
CGTTGCT200.00694327452.534
AACGCTG200.00694327452.569
AGTCGTG200.00694327452.531
CGTATCA1350.051.85185256
TAGATCT1450.050.68965539
GTATCAT1450.048.2758657
CATTGAA451.6784179E-646.66666426
TGAACTA451.6784179E-646.66666429
AATGTAT451.6784179E-646.66666446
CGCCGTA1650.044.54545653
AGATTCA503.4657241E-642.021
CAATGTA503.4657241E-642.045
CTAGGTC503.4657241E-642.033
TCATTAA1550.040.6451660
ATCATTA1550.040.6451659
Produced by FastQC (version 0.11.5)