FastQCFastQC Report
Wed 25 May 2016
SRR1779632_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779632_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences192188
Sequences flagged as poor quality0
Sequence length76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGT84364.3894519949216395No Hit
TCTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCG15550.8091035860719712No Hit
ATTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGT11300.5879659500072846No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10390.5406164796969635No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCC7840.4079338980581514No Hit
TCTTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCC2350.12227610464753261No Hit
ATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGTCTT2100.10926800840843341RNA PCR Primer, Index 47 (95% over 21bp)
CTAATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGT2070.1077070368597415No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAATGC207.890295E-570.0385441
GTCATTC150.002221814670.0020859
AGGGGGG10100.054.04694470
TAAACCG200.00694960452.48795
GCGTTGT200.00694960452.487923
CAATCGT200.00694960452.487928
ATCGATA200.00694960452.487923
TAATCGA200.00694960452.487921
CCGTCTT16750.050.39905547
CGTCTTC17500.049.84039748
CGTATGC17950.049.74882541
GTATGCC17400.049.7242142
ATGCCGT17350.049.66561544
TATGCCG17400.049.522943
GCCGTCT17050.049.5122746
TCTTCTG17800.049.36824850
TCGTATG18550.049.2595740
GTCTTCT17850.049.22996549
TGCCGTC17450.049.18026445
CTCGTAT18350.049.0332939