FastQCFastQC Report
Wed 25 May 2016
SRR1779631_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779631_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences410830
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG166664.056665774164496No Hit
CTTATACACATCTGACGCTGCCGACGATCTACTCTGTGTAGATCTCGGTG7290.1774456587883066No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTTA200.006949207352.49936313
GCCGTAT1000.052.49936354
CGTATCA1350.038.88841656
CCGTATC1350.038.88841655
CGCCGTA1550.038.38662753
TCTCGGT1550.036.12859343
TGGACTT400.002417774234.9995775
CGTTAAA501.7086965E-434.99957717
TATACCG652.1009258E-532.3072976
TGGTCGC1850.032.1617749
TGCTAGC450.00430296331.11452157
CCGATAC450.00430552731.11073139
GTTAAGC450.00430552731.11073156
GTAGATC1850.030.26990138
ATCTCGG1750.029.99963642
AGATCTC1750.029.99963640
TAGATCT1800.029.1663139
TCGGTGG1950.028.71759845
GTATCAT1601.8189894E-1228.44061757
TGGCATA500.00720548927.9996630