FastQCFastQC Report
Wed 25 May 2016
SRR1779631_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779631_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences410830
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGT93702.280748728184407No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCG16730.4072243993866076No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG13130.31959691356522163No Hit
ATTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGT11810.28746683543071344No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCC10190.24803446681108976No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACGTA150.002224911569.99184438
AACGTAT252.3891607E-456.0002939
ACGTATG509.804353E-1056.0002940
CGTATGA452.6822818E-854.4845341
AGGGGGG12850.052.5491770
TCGTATG19300.050.95881740
TATGCCG18800.050.88048643
CCGTCTT17700.050.29361347
ATCTCGT18650.050.28904337
CGTCTTC18200.050.25988448
CGTATGC19600.050.2155241
CTCGTAT19200.049.76588439
CTTGAAA18850.049.5758657
TCTCGTA19350.049.3740938
GTATGCC19550.048.92855542
ATGCCGT19350.048.89699644
ATATCGT651.8553692E-1048.4558937
TCTTCTG18950.048.43793550
TGCCGTC19350.047.9973545
GTCTTCT19000.047.94168549
Produced by FastQC (version 0.11.5)