FastQCFastQC Report
Wed 25 May 2016
SRR1779630_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779630_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences289837
Sequences flagged as poor quality0
Sequence length76
%GC61

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG154675.336447727515811No Hit
CTTATACACATCTGACGCTGCCGACGATCTACTCTGTGTAGATCTCGGTG6420.22150381076260103No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACCGTT200.00694681952.57
TCGTAAT200.00694681952.550
GTATAAG404.5862635E-543.751
TTAGGTG400.002416459135.049
ATTAGGT400.002416459135.048
TAGGTGC400.002416459135.050
TGTATAA501.707335E-435.02
TCCAATT400.002416459135.044
ATCATTA1201.8189894E-1235.059
CGTATCA1253.6379788E-1233.60000256
GCCGTAT1357.2759576E-1231.1111154
TATCATT1401.2732926E-1130.058
TCATTAA1401.2732926E-1130.060
TAGATCT1551.8189894E-1229.3548439
CATTAAA1451.8189894E-1128.96551761
AAGAGAC853.9455736E-628.823535
GTACAAA500.00720161527.9999981
CCTAGTA500.00720161527.99999865
CTTCCAA500.00720161527.99999842
TCTTATG500.00720161527.99999863
Produced by FastQC (version 0.11.5)