FastQCFastQC Report
Wed 25 May 2016
SRR1779630_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779630_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences289837
Sequences flagged as poor quality0
Sequence length76
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGT89033.071726522148656No Hit
TCTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCG15450.5330582361810259No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11200.38642409354223234No Hit
ATTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGT10700.36917301793766844No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCC9760.3367409958010882No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTAA150.00222428669.9915516
AGGGGGG10050.056.75930870
TTACCGT200.006950136352.493661
TATCGTA552.2791937E-950.9029438
CTTGAAA18350.050.3655257
TATGCCG17500.050.01985543
CTCGTAT18350.049.77639
CCGTCTT17050.049.6971447
TTGAAAA18600.049.68856458
TCGTATG18950.049.6859540
TCTGCTT18050.049.659653
GTATGCC17700.049.45465542
CGTATGC18550.049.44505741
TCTCGTA18500.049.3724138
TTCTGCT18250.048.7232652
CTTCTGC18400.048.70657751
TGAAAAA19400.048.54181759
GTCTTCT18300.048.39883849
TGCTTGA18750.048.35751355
TCTTCTG18250.048.14778550
Produced by FastQC (version 0.11.5)